LATEST NEWS older news

What makes mutants of the lipid transporting protein ApoA-I  turn into amyloids?  Read more here or here.

A collaboration with Prof. Thomas Smithgall from the University of Pittsburgh School of Medicine reveals how an inhibitor blocks the HIV-1 Nef protein from activating the hematopoetic cell kinase (Hck).            read more

The impact of membranes on peripheral membrane protein conformation and dynamics can be studied with our new methods using Langmuir monolayers.  See Paper #1 and its sister, Paper #2.

The analytical aspects of HX MS are reviewed in a new article in the Annual Reviews of Analytical Chemistry.  A key attribute, replication, is further discussed in an editorial in Bioanalysis.    more


The laboratory of John R. Engen at Northeastern University performs research at the interface of Analytical Chemistry, Biochemistry & Structural Biology.


We use hydrogen exchange and mass spectrometry (HX MS) as our core technology to probe protein conformation, conformational changes, dynamics, protein folding and the effects of binding.

In the past 14 years, we have applied HX MS to the study of nearly 100 proteins.