RESEARCH:
HYDROGEN EXCHANGE MASS SPECTROMETRY (HX MS)

Research in the Engen Laboratory centers around hydrogen exchange (HX) mass spectrometry (MS). The technique is described in more detail within the links of this section.

We not only develop new, better and easier ways of doing HX MS, but we apply these tools to a vast number of different types of proteins.  Some of our projects are listed below.  We have many collaborations wherein we primarily apply HX MS to the study of proteins related to disease.  For more details, see also the section on publications

Tutorial:

Background / Theory
  What is it?
  How does it work?
  What is it used for?
  Exchange theory
  Mass spectrometry
Methods
  Labeling methods
  Ionization methods
  Basic experiment
  Controls/calculations

Projects:

Src-family & Tec-family kinases
Viral proteins: HIV Nef, HIV Vif
Abl kinase
Membrane proteins
Allostery and protein conformation
Biopharmaceticals
HX MS methods development
UPLC at low temperatures
HX-Express software
Acid proteases
Ion-mobility to raise peak capacity
Digestion under pressure

Other Research Links:

Collaborators
Our laboratory and associated equipment
Research news